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genomic pcr primer design  (PrimerDesign Inc)

 
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    PrimerDesign Inc genomic pcr primer design
    POLQ knockout by CRISPR-Cas9 induces cell cycle arrest in high-risk neuroblastoma LA1–55n cells. ( A ) Genomic <t>PCR</t> results shows POLQ targeting in clone KO-Q6 and KO-Q63. The upper panel diagram of genomic <t>PCR</t> <t>Primer</t> design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction). Lower left panel show left integration junction PCR products and lower right panel show right integration junction PCR products amplified from blasticidin selected colonies in 1.5 % agarose gel. ( B ) Immunoblot validation of POLQ expression knockout in KO-Q6 and KO-Q63 colonies. ( C ) mRNA POLQ expression was measured in LA1–55n control, KO-Q6 and KO-Q63 colonies (*** p < 0.001) ( D ) Cell growth time was observed using MTS/PMS assay in Control and KO (KO-Q6 and KO-Q63) cells. Cell growth was reduced in POLQ KO groups when compared to Control (**** p < 0.0001) ( E ) The ability to form colonies of LA1–55n Control and POLQ KO (KO-Q6 and KO-Q63) was investigated. The number of colonies in POLQ KO (KO-Q6 and KO-Q63) groups were significantly less than the control group.
    Genomic Pcr Primer Design, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic pcr primer design/product/PrimerDesign Inc
    Average 90 stars, based on 1 article reviews
    genomic pcr primer design - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "POLQ mediated end-joining promotes DNA damage tolerance in neuroblastoma"

    Article Title: POLQ mediated end-joining promotes DNA damage tolerance in neuroblastoma

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2025.102433

    POLQ knockout by CRISPR-Cas9 induces cell cycle arrest in high-risk neuroblastoma LA1–55n cells. ( A ) Genomic PCR results shows POLQ targeting in clone KO-Q6 and KO-Q63. The upper panel diagram of genomic PCR Primer design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction). Lower left panel show left integration junction PCR products and lower right panel show right integration junction PCR products amplified from blasticidin selected colonies in 1.5 % agarose gel. ( B ) Immunoblot validation of POLQ expression knockout in KO-Q6 and KO-Q63 colonies. ( C ) mRNA POLQ expression was measured in LA1–55n control, KO-Q6 and KO-Q63 colonies (*** p < 0.001) ( D ) Cell growth time was observed using MTS/PMS assay in Control and KO (KO-Q6 and KO-Q63) cells. Cell growth was reduced in POLQ KO groups when compared to Control (**** p < 0.0001) ( E ) The ability to form colonies of LA1–55n Control and POLQ KO (KO-Q6 and KO-Q63) was investigated. The number of colonies in POLQ KO (KO-Q6 and KO-Q63) groups were significantly less than the control group.
    Figure Legend Snippet: POLQ knockout by CRISPR-Cas9 induces cell cycle arrest in high-risk neuroblastoma LA1–55n cells. ( A ) Genomic PCR results shows POLQ targeting in clone KO-Q6 and KO-Q63. The upper panel diagram of genomic PCR Primer design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction). Lower left panel show left integration junction PCR products and lower right panel show right integration junction PCR products amplified from blasticidin selected colonies in 1.5 % agarose gel. ( B ) Immunoblot validation of POLQ expression knockout in KO-Q6 and KO-Q63 colonies. ( C ) mRNA POLQ expression was measured in LA1–55n control, KO-Q6 and KO-Q63 colonies (*** p < 0.001) ( D ) Cell growth time was observed using MTS/PMS assay in Control and KO (KO-Q6 and KO-Q63) cells. Cell growth was reduced in POLQ KO groups when compared to Control (**** p < 0.0001) ( E ) The ability to form colonies of LA1–55n Control and POLQ KO (KO-Q6 and KO-Q63) was investigated. The number of colonies in POLQ KO (KO-Q6 and KO-Q63) groups were significantly less than the control group.

    Techniques Used: Knock-Out, CRISPR, Amplification, Agarose Gel Electrophoresis, Western Blot, Biomarker Discovery, Expressing, Control



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    POLQ knockout by CRISPR-Cas9 induces cell cycle arrest in high-risk neuroblastoma LA1–55n cells. ( A ) Genomic <t>PCR</t> results shows POLQ targeting in clone KO-Q6 and KO-Q63. The upper panel diagram of genomic <t>PCR</t> <t>Primer</t> design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction). Lower left panel show left integration junction PCR products and lower right panel show right integration junction PCR products amplified from blasticidin selected colonies in 1.5 % agarose gel. ( B ) Immunoblot validation of POLQ expression knockout in KO-Q6 and KO-Q63 colonies. ( C ) mRNA POLQ expression was measured in LA1–55n control, KO-Q6 and KO-Q63 colonies (*** p < 0.001) ( D ) Cell growth time was observed using MTS/PMS assay in Control and KO (KO-Q6 and KO-Q63) cells. Cell growth was reduced in POLQ KO groups when compared to Control (**** p < 0.0001) ( E ) The ability to form colonies of LA1–55n Control and POLQ KO (KO-Q6 and KO-Q63) was investigated. The number of colonies in POLQ KO (KO-Q6 and KO-Q63) groups were significantly less than the control group.
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    Image Search Results


    POLQ knockout by CRISPR-Cas9 induces cell cycle arrest in high-risk neuroblastoma LA1–55n cells. ( A ) Genomic PCR results shows POLQ targeting in clone KO-Q6 and KO-Q63. The upper panel diagram of genomic PCR Primer design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction). Lower left panel show left integration junction PCR products and lower right panel show right integration junction PCR products amplified from blasticidin selected colonies in 1.5 % agarose gel. ( B ) Immunoblot validation of POLQ expression knockout in KO-Q6 and KO-Q63 colonies. ( C ) mRNA POLQ expression was measured in LA1–55n control, KO-Q6 and KO-Q63 colonies (*** p < 0.001) ( D ) Cell growth time was observed using MTS/PMS assay in Control and KO (KO-Q6 and KO-Q63) cells. Cell growth was reduced in POLQ KO groups when compared to Control (**** p < 0.0001) ( E ) The ability to form colonies of LA1–55n Control and POLQ KO (KO-Q6 and KO-Q63) was investigated. The number of colonies in POLQ KO (KO-Q6 and KO-Q63) groups were significantly less than the control group.

    Journal: Translational Oncology

    Article Title: POLQ mediated end-joining promotes DNA damage tolerance in neuroblastoma

    doi: 10.1016/j.tranon.2025.102433

    Figure Lengend Snippet: POLQ knockout by CRISPR-Cas9 induces cell cycle arrest in high-risk neuroblastoma LA1–55n cells. ( A ) Genomic PCR results shows POLQ targeting in clone KO-Q6 and KO-Q63. The upper panel diagram of genomic PCR Primer design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction). Lower left panel show left integration junction PCR products and lower right panel show right integration junction PCR products amplified from blasticidin selected colonies in 1.5 % agarose gel. ( B ) Immunoblot validation of POLQ expression knockout in KO-Q6 and KO-Q63 colonies. ( C ) mRNA POLQ expression was measured in LA1–55n control, KO-Q6 and KO-Q63 colonies (*** p < 0.001) ( D ) Cell growth time was observed using MTS/PMS assay in Control and KO (KO-Q6 and KO-Q63) cells. Cell growth was reduced in POLQ KO groups when compared to Control (**** p < 0.0001) ( E ) The ability to form colonies of LA1–55n Control and POLQ KO (KO-Q6 and KO-Q63) was investigated. The number of colonies in POLQ KO (KO-Q6 and KO-Q63) groups were significantly less than the control group.

    Article Snippet: The upper panel diagram of genomic PCR Primer design, two set of primer were utilized to detect the left and right integration junctions (LF, LR: Forward and reverse PCR primer to amplify the left integration junction; RF, RR: Forward and reverse PCR primer to amplify the right integration junction).

    Techniques: Knock-Out, CRISPR, Amplification, Agarose Gel Electrophoresis, Western Blot, Biomarker Discovery, Expressing, Control